Development of Monoclonal-antibody-ELISA, -DOT-BLOT and -DIP-STICK Immunoassays for Humicola lanuginosa in Rice
نویسندگان
چکیده
منابع مشابه
Development of monoclonal-antibody-ELISA and -DIP-STICK immunoassays for Penicillium islandicum in rice grains.
Monoclonal antibodies (mAbs) were raised against Penicillium islandicum, a fungus commonly isolated from stored rice grains in South-East Asia. Mice were immunized by a direct, simple method; fresh cell-free surface washings from a solid agar slant culture were injected directly into the peritoneum without prior concentration. Hybridoma supernatants were screened by ELISA. Most of the mAbs rais...
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Rice tungro disease (RTD) is one of the most destructive diseases of rice in South and Southeast Asia. RTD is routinely detected based on visual observation of the plant. However, it is not always easy to identify the disease in the field as it is often confused with other diseases or physiological disorders. Here we report the development of two serological based assays for ease of detection o...
متن کاملDevelopment of enzyme linked, tissue blot and dot blot immunoassays for plant virus detection.
Immunoassays are among the most powerful and useful techniques for analysis of biological materials. There are numerous variations in which immunoassays can be performed. Coupled with enzyme, using chromogenic substrates, the enzyme immunoassay technique is used to trace the target antigen in tissues. The technique also is used to measure the concentration of antigen in tissue extracts. This ch...
متن کاملDevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
متن کاملDetection of circulating antibody of Parastrongylus cantonensis in sera with eosinophilic meningitis by dot-blot ELISA.
BACKGROUND Eosinophilic meningitis caused by Parastrongylus cantonensis, the rat lung worm is a major public health problem in Thailand. Humans acquire this parasite by eating raw food containing infective Parastrongyliasis is dificult to make because identification of parasite materials by biopsy or chance finding is rarely possible. OBJECTIVE Develop alternative approaches of Parastrongylus...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1989
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-135-2-361